House dust mites as potential carriers for IgE sensitization to bacterial antigens

Autores/as

  • Sheron Dzoro Medical University of Vienna, Centre for Pathophysiology, Infectiology and Immunology, Department of Pathophysiology and Allergy Research, Vienna
  • Irene Mittermann Medical University of Vienna, Centre for Pathophysiology, Infectiology and Immunology, Department of Pathophysiology and Allergy Research, Vienna
  • Yvonne Resch Medical University of Vienna, Centre for Pathophysiology, Infectiology and Immunology, Department of Pathophysiology and Allergy Research, Vienna
  • Susanne Vrtala Medical University of Vienna, Centre for Pathophysiology, Infectiology and Immunology, Department of Pathophysiology and Allergy Research, Vienna
  • Marion Nehr Medical University of Vienna, Clinical Institute of Laboratory Medicine, Division of Clinical Microbiology, Vienna
  • Alexander M. Hirschl Medical University of Vienna, Clinical Institute of Laboratory Medicine, Division of Clinical Microbiology, Vienna
  • Gustav Wikberg Karolinska University Hospital, Dermatology and Venereology Unit, Stockholm
  • Lena Lundeberg Karolinska University Hospital, Dermatology and Venereology Unit, Stockholm
  • Catharina Johansson Karolinska Södersjukhuset, Institutet and Sachs’ Children and Youth Hospital, Department of Clinical Science and Education, Stockholm
  • Annika Scheynius Karolinska Södersjukhuset, Institutet and Sachs’ Children and Youth Hospital, Department of Clinical Science and Education, Stockholm
  • Rudolf Valenta Medical University of Vienna, Centre for Pathophysiology, Infectiology and Immunology, Department of Pathophysiology and Allergy Research, Vienna

Palabras clave:

Atopic dermatitis, House dust mites, Bacterial antigens

Resumen

Background: IgE-reactivity to antigens from gram-positive and negative bacteria is common in patients suffering from respiratory and skin manifestations of allergy, but the routes and mechanisms of sensitisation are not fully understood. The analysis of the genome, transcriptome and microbiome of house dust mites has shown that Staphylococcus aureus and Escherichia coli species are abundant bacteria within the house dust mites microbiome. Therefore, our aim was to investigate if house dust mites are carriers of bacterial antigens leading to IgE sensitisation in patients suffering from atopic dermatitis.

Methods: Plasma samples from atopic dermatitis patients (n = 179) were analysed for IgE-reactivity to a comprehensive panel of micro-arrayed house dust mites allergen molecules and to Staphylococcus aureus and Escherichia coli by IgE immunoblotting. Antibodies specific for Staphylococcus aureus and Escherichia coli antigens were tested for reactivity to nitrocellulose-blotted extract from purified house dust mites bodies and the IgE-reactive antigens were detected by IgE-immunoblot inhibition experiments. IgE antibodies directed to bacterial antigens in house dust mites were quantified by IgE ImmunoCAPTM inhibition experiments.

Results: IgE-reactivity to bacterial antigens was significantly more frequent in atopic dermatitis patients sensitised to house dust mites than in atopic dermatitis patients without house dust mites sensitisation. Staphylococcus aureus and Escherichia coli antigens were detected in immune-blotted house dust mites extract and the presence of IgE-reactive antigens in house dust mites was demonstrated by qualitative and quantitative IgE inhibition experiments.

Conclusions: House dust mites may serve as carriers of bacteria responsible for the induction of IgE sensitisation to microbial antigens.

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