Supplementary Files
Keywords
Acute lymphocytic leukemia
Chronic myelogenous Leukemia
Venozolan mestizo population
Abstract
Background: The HLA complex is involved in the pathogenesis of leukemia.
Objectives: The presence of class II HLA alleles DRB1 *, DQB1 *, DPA1 *, and DPB1 * was evaluated in 47 patients with acute lymphoblastic leukemia (ALL) and 48 with chronic myeloid leukemia (CML) for comparison with 48 healthy volunteers in Zulia, Venezuela, and to evaluate potential associations of HLA with leukemia.
Methods: Low- and high-resolution PCR-SSP was used for class II HLA regions DRB1 *, DQB1 *, DPA1 *, and DPB1 * following the instructions of KIT Olerup SSP Genovision.
Results: Alleles HLA-DRB1*14, especially DRB1*14:21, -DPA1*1:06, -DPA1*01:03,-DPA1*02:01, and the haplotypes HLA-DPA1*01:03-DPB1*04:01, DPA1*01:03-DPB1*02:01, DPA1*01:03-DPB1*99:01, -DRB1*14-DPA1*01:03, -DRB1*15-DPA1*01:03 were associated with CML (RR > 3); alleles HLA-DRB1*13, -DQB1*02, -DPA1*01:05, -DPA1*01:09 and the haplotypes HLA-DPA1*01:09-DPB1*02:01, DPA1*01:09-DPB1*04:01 were protective (RR < 1). Alleles HLA-DQB1*04, -DQB1*05, -DPA1*1:06, -DPA1*01:07, -DPA1*1:08 had a positive association with ALL. Alleles HLA-DPA1*01:09, -DPA1*02:01, -DPB1*02:01, -DPB1*03:01 and the haplotypes HLA-DPA1*01:03-DPB1*04:02, -DPA1*01:09-DPB1*02:01, -DPA1*01:09-DPB1*04:01, -DPA1*02:01-DPB1*04:02 were negatively associated.
Conclusions: The absence of associations with HLA-DRB1 * region in ALL and other association patterns identified suggest marked differences in the pathogenesis of leukemia, which suggests possible deficiencies in antigen presentation for ALL or potential effects of molecular mimicry in CML.
References
Tirado-Gomez LL, Mohar Betancourt A. Epidemiología de las neoplasias hemato-oncológicas. Cancerologia. 2007;2:109-20. [Citado 2015 May 03]. Disponible en: http://www.incan.org.mx/revistaincan/elementos/documentosPortada/1193426695.pdf
World Health Organization [Internet]. Colin DM, Boschi-Pinto C, Lopez AD, Murray CJL. Cancer incidence, mortality and survival by site for 14 regions of the world. Global Programme on Evidence for Health Policy Discussion Paper No. 13. 2001. [Citado 2014 Oct 10] Disponible en: http://www.who.int/entity/healthinfo/paper13.pdf
Rodríguez M, Cardona AF, Grajales MA, Enciso L, Ruiz G, Yepes A, et al. Leucemia mieloide crónica en crisis blástica bases moleculares y diagnóstico. Rev Venez Oncol. [Internet]. 2007; 19(4):287-296 [Citado 2016 Jul 02]. Disponible en: http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0798-05822007000400003&lng=es.
Capote Negrin LG. Aspectos epidemiológicos del cáncer en Venezuela. Rev. venez. oncol. [Internet]. 2006;18(4):269-281 [Citado 2016 Jul 02]. Disponible en: http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0798-05822006000400011&lng=e
Gorodezky C. Genetic difference between Europeans and Indians. Allergy Proc. 1992;13(5):243-250.
Villalobos C, Rivera S, Weir-Medina J, Hassanhi M, Montiel M, Gonzales R. Asociación HLA clase I y leucemia en pacientes mestizos del estado Zulia, Venezuela.. Invest Clin. 2003;44(4):283-289.
Ramiro GFJ. Declaración de Helsinki de la Asociación Médica Mundial. Principios éticos para las investigaciones médicas en seres humanos. 2005. Disponible en: http://www.bioeticaweb.com/index2.php?option=com_content&do_pdf=1&id=896 (citado en abril del 2011).
Briceño E, Suárez E, Michelangeli C, Feliciangeli D, Otaiza E, Mendible JC, et al. Código de biotética y bioseguridad. 2ª. edición. Venezuela: Ministerio de Ciencia y Tecnología (FONACIT); 2002. (Citado 2011 Feb 20). Disponible en: http://www.miproyecto.gov.ve/anexos/bioetica.pdf
Miller SA, Dkyes DD, Polesky HF. A simple salting out procedure for extracting DNA from human cell. Nucleic Acids Res. 1988;16(3):1215.
Raymond M, Rousset F, GENEPOP (version 1.2) population genetics software for exact test and ecumerism. J Herad. 1995;86:248-249.
Excoffier L, Laval G, Schneider S. Arlequin ver 3.0: An integrated software package for population genetics data analysis. Evol Bioinform Online 2005;1:47-50.
McLaughlin J, Chianese E, Wittte ON. In vitro transformation of immature hematopoietic cells by the p210 bcr/abl oncogen product of the Philadelphia chromosome. Proc NatlAcad Sci USA. 1987;84:6558-6562.
Yasukawa M, Ohminami H, Kojima K, Inokuchi K, Nishimura Y, Fujita S. Analysis of HLA-DRB1 alleles in Japanese patients with chronic myelogenous leukemia. Am J Hematol 2000;63(2):99-101.
Yasukawa M, Ohminami H, Kojima K, Hato T, Hasegawa A, Takahashi T, et al. HLA class II-restred antigen presentation of endogenus bcr-abl fusion protein by chronic myelogenus leukemia-derived dendritic cells to CD4+ T lymphocytes. Blood. 2001;98(5):1498-1505.
Dorak MT, Burnett AK. Molecular mimicry of an HLA-DR53 epitope by viruses [letter]. Immunol Today. 1994;15:138-139.
Dorak MT. The implications for childhood leukaemia of infection with adenovirus. Trends Microbiol. 1996;4:60-63.
Dorak MT, Lawson T, Machulla HKG, Darke C, Mills KI, Burnett AK. Unravelling an HLA-DR association in childhood acute lymphoblastic leukemia. Blood. 1999;94:694-700.
Dorak MT, Oguz FS, Yalman N, Diler AS, Kalayoglu S, Anak S, Sargin D, Carin M. A male-specific increase in the HLA-DRB4(DR53) frequency in high-risk and relapsed childhood ALL. Leuk Res. 2002;26(7):651-656.
Dorak MT. MHC and leukemia. 2007. [Citado 2012 Ene 07]. Disponible en: http://www.dorak.info/mhc/mhcleuk.html.
Gragert L, Fingerson S, Albrecht M, Maiers M, Kalaycio M, Hill BT. Fine-mapping of HLA associations with chronic lymphocytic leukemia in US populations Blood. 2014;124(17):2657-2665.
Hosking FJ, Leslie S, Dilthey A, Moutsianas L, Wang Y, Dobbins SE, et al. MHC variation and risk of childhood B-cell precursor acute lymphoblastic leukemia. Blood. 2011;117:1633-40. doi:10.1182/blood-2010-08-301598.
Kang H, Wilson CS, Harvey RC, Chen IM, Murphy MH, Atlas SR,, et al. Gene expression profiles predictive of outcome and age in infant acute lymphoblastic leukemia: a Children’s Oncology Group study. Blood. 2012;119(8):1872-81. doi: 10.1182/blood-2011-10-382861.
Godoresky C, Flores H, Alaez C, Sánchez D, Munguía A, Rodríguez A, et al. Spectrum of hla association with acute lymphoblastic leukemia in Mexican patients. Human Immunology. 2014;75 (Suppl):123.
Echeverría M, Rivera S, Márquez G, Carrillo, Z, Parra Y, Villalobos C. Patrones diferenciales de distribución de los alelos y genotipos HLA-DPA1* en asociación con leucemias linfoides agudas y leucemias mieloides crónica. Inmunologia. 2010;29(4):115-118.
Rivera PS, Echeverría M, Márquez G, Villalobos C, Pereira N, De Salvo L, et al. Asociación HLA-DR/DQ/DPA1 con leucemias linfoides agudas (LLA) y leucemias mieloides crónica (LMC) en la población mestiza del estado Zulia, Venezuela (estudio preliminar). Ciencia. 2007;15(4):365-372.
Wang XJ, Ai XF, Sun HY, Wang F, Xu SC, Wang ZQ. Relation of HLA-DRB1*15 with pathogenesis in 162 childhood cases of acute lymphoblastic leukemia. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2009;17:1507-1510.
Taylor GM, HussainA, Verhage V, et al. Strong association of the HLADP6 supertype with childhood leukaemia is due to a single allele, DPB1*0601. Leukemia. 2009;23:863-9. doi:10.1038/leu.2008.374.
Urayama K, Chokkalingam A, Metayer C, Ma X, Selvin S, Barcellos L, et al. HLA-DP genetic variation, proxies for early life immune modulation and Childhood acute lymphoblastic leukemia risk. Blood. 2012;120:3039-3047.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright (c) 2016 Revista Alergia México