Supplementary Files
Keywords
TNF-α
TNFα polymorphism
TNF-α-308 polymorphism
Prostate specific antigen
How to Cite
Plaudit
Abstract
Background: Prostate cancer is the third cause of cancer death in men in the Western hemisphere and the second cause of cancer death in Zulian men from Venezuela.
Objective: To determine whether polymorphisms 308 and 238 of the tumor necrosis factor alpha (TNF-α) gene are associated with prostate cancer.
Methods: The DNA that was extracted from the peripheral blood of 40 patients with prostatic specific antigen and 40 controls was amplified by PCR plus digestion with enzymes NcoI and MspI.
Results: In the patients, genotypes of the TNF-α-238 polymorphism were observed in 90% GG and 10% GA; in controls, in 97.5% GG and 2.5% GA, odds ratio (OR) = 4,000 for GA. In the patients, genotypes of TNF-α-308 polymorphism were identified in 85% GG and 15% GA, and in controls in 72.5% GG and 27.5% GA, OR = 0.545 for GA and 1.172 for GG. The allelic frequencies for TNF-α-238 in patients were 95% for G and 5% for A; in controls, 98.75% for G and 1.25% for A, with OR = 4,000 for A. The allelic frequencies for TNF-α-308 in the patients were 92.5% for G and 7.5% for A.
Conclusions: There weren’t any statistically significant associations. The allele A of the TNF-α-238 polymorphism resulted in a considerable risk factor for prostate cancer.
References
Ministerio del Poder Popular para la Salud. Anuario de mortalidad de la República Bolivariana de Venezuela. Venezuela: Ministerio del Poder Popular para la Salud; 2014.
Scher HI. Enfermedades benignas y malignas de la próstata. En: Jameson JL, Fauci AS, Kasper DL, Hauser SL, Longo DL, Loscalzo J. Harrison´s principles of internal medicine. Décima novena edición. EE. UU.: McGraw-Hill; 2015.
Zhu LB, Zhao ST, Xu TZ, Wang H. Tumor necrosis factor-α-induced a disintegrin and metalloprotease 10 increases apoptosis resistance in prostate cancer cells. OncolLett. 2014;7(3):897-901. DOI: 10.3892/ol.2014.1810
Thun M, Thun M, Jemal A, Siegel R, Ward E, Hao Y, et al. Cancer statistics. CA Cancer J Clin. 2008;58(2):71-96.
Kasper D, Braunwald E, Fauci A, Hauser S, Longo D, Jameson J. Harrison’s Principles of Internal Medicine. EE. UU.: McGraw-Hill; 2008.
Beers M, Porter R, editores. El manual Merck. EE. UU.: Elsiever; 2007.
MacLennan G, Eisenberg R, Fleshman R, Taylor J, Fu P, Resnick M, et al. The influence of chronic inflammation in prostatic carcinogenesis: a 5-year follow-up study. J Urol. 2006;176(3):1012-1016. DOI: 10.1016/j.juro.2006.04.033
Abbas A, Lichtman A, Pillai S. Cellular and molecular immunology. Saunders; 2007.
Sáenz-López P, Carretero R, Cózar J, Romero J, Canton J, Vilchez JR, et al. Genetic polymorphisms of RANTES, IL1-A, MCP-1 and TNF-A genes in patients with prostate cancer. BMC Cancer. 2008;8:382. DOI: 10.1186/1471-2407-8-382
Zabaleta J, Lin HY, Sierra RA, Hall MC, Clark PE, Sartor OA, et al. Interactions of cytokine gene polymorphisms in prostate cancer risk. Carcinogenesis. 2008;29(3):573-578. DOI: 10.1093/carcin/bgm27
Lippitz BE. Cytokine patterns in patients with cancer: a systematic review. Lancet Oncol. 2013;14(6):218-228. DOI: 10.1016/S1470-2045(12)70582-X
Wu HC, Chang CH, Chen HY, Tsai FJ, Tsai JJ, Chen WC. p53 gene codon 72 polymorphism but not tumor necrosis factor-alpha gene is associated with prostate cancer. Urol Int. 2004;73(1):41-46. DOI: 10.1159/000078803
McCarron S, Edwards S, Evans PR, Gibbs R, Dearnaley DP, Dowe A, et al. Influence of cytokine gene polymorphisms on the development of prostate cancer. Cancer Res. 2002;62(12):3369-3372. Disponible en: http://cancerres.aacrjournals.org/content/canres/62/12/3369.full.pdf
Danforth KN, Rodríguez C, Hayes RB, Sakoda LC, Huang WY, Yu K, et al. TNF polymorphisms and prostate cancer risk. Prostate. 2008;1;68(4):400-407. DOI: 10.1002/pros.20694
Anaya J. Descripción molecular del TNF-α. Reumatologia. 2003;19(2):112-120.
Miller SA, Dikes DD, Polesky HF. A simple salting-out procedure for extracting DNA from human nucleated cells. Nucl Acid Res. 1987;16(3):1215.
Fargion S, Valenti L, Dongiovanni P, Ludovica A. TNFα promotor polymorphism. En: Corti A, Ghezzi P. Tumor necrosis factor. Methods and protocols. EE. UU.: Humana Press; 2004.
SPSS 20 statistical analysis, data mining, text analytics, data collection, collaboration & deployment. EE. UU.: International Business Machines; 2012.
Wang J, Cao C, Luo H, Xiong S, Xu Y, Xiong W. Tumour necrosis factor alpha-308G/A polymorphism and risk of the four most frequent cancers: a meta-analysis. Int J Immunogenet. 2011;38:311-320.
Mangyuan W, Zhenwei H, Jiaming L, Banghua L. TNFα gene–308 G/A polymorphism and the risk of prostate cancer: a meta-analysis. Chin J Evid-based Med. 2012;8:1030-1034.
Ma L, Zhao J, Li T, He Y, Wang J, Xie L, et al. Association between tumor necrosis factor-alpha gene polymorphisms and prostate cancer risk: a meta-analysis. Diagn Pathol. 2014;25(9):74. DOI: 10.1186/1746-1596-9-74
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