Vol. 63 No. 1 (2016), Original articles
Vol. 63 No. 1 (2016)

Sensitivity and specificity of prick skin test with two concentrations of standardized extract of Culex quinquefasciatus in allergic children

Original articles
Published 2016-03-01
Raúl Lázaro Castro-Almarales
Centro Nacional de Biopreparados, La Habana
Mirta Álvarez-Castelló
Facultad de Ciencias Médicas General Calixto García Íñiguez, Hospital Universitario, Servicio de Alergología, La Habana
Mercedes Ronquillo-Díaz
Facultad de Ciencias Médicas General Calixto García Íñiguez, Hospital Universitario, Servicio de Alergología, La Habana
José S Rodríguez-Canosa
Facultad de Ciencias Médicas General Calixto García Íñiguez, Hospital Universitario, Servicio de Alergología, La Habana
Mayda González-León
Policlínico Docente Pedro Fonseca, Punta Brava, La Lisa, La Habana
Bárbara I Navarro-Viltre
Hospital General de Cataluña, Barcelona
Daniel Betancourt-Mesia
Hospital Héroes del Baire, Nueva Gerona, Isla de la Juventud
Irene Enríquez-Domínguez
Facultad de Ciencias Médicas General Calixto García Íñiguez, Hospital Universitario, Servicio de Alergología, La Habana
Mary Carmen Reyes-Zamora
Hospital Policlínico Docente Rosa Elena Simeón, Bejucal, Mayabeque y ISCMPC Victoria de Girón, La Habana
Yunia Oliva-Díaz
Centro Nacional de Biopreparados, La Habana
Mayteé Mateo-Morejón
Centro Nacional de Biopreparados, Mayabeque
Alexis Labrada-Rosado
Centro Nacional de Biopreparados, Mayabeque
PubMed

Keywords

sensitivity
specificity
skin prick test
Culex quinquefasciatus

Abstract

BACKGROUND: Diagnostic options for immune reactions to mosquito bites are limited. In Cuba, IgE-mediated reactions are frequently related to Culex quinquefasciatus bite.

OBJECTIVE: To determine the sensitivity and specificity of skin prick test with two doses of standardized extract in nitrogen protein units (PNU) of Culex quinquefasciatus (BIOCEN, Cuba).

MATERIAL AND METHOD: An analytical study was conducted on 100 children between 2 and 15 years old. Fifty atopic patients with a history of allergy to mosquito bite and positive specific serum IgE Culex quinquefasciatus and fifty atopic patients without a history of allergy to mosquito bite and negative specific serum IgE to Culex quinquefasciatus. Skin prick tests (SPT) were performed by duplicates on the forearms of the patients. Investigated doses were 100 PNU/mL and 10 PNU/mL.

RESULTS: SPT with the highest concentration obtained a mean wheal size of 22.09 mm2and for lower doses of 8.09 mm2, a statistically significant difference (p=0.001, Student’s t test). Positive skin test correlated in 100% of patients with the presence of specific IgE. Testing with both doses showed a 94% of specificity and 88% of sensitivity.

CONCLUSION: The diagnostic accuracy of SPT using both doses of standardized extract was similar, which justifies its use for diagnosis of sensitization to Culex quinquefasciatusin patients with symptoms of allergy to mosquito bite.

PubMed

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